Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

Artigo - Open Access.

Idioma principal

AGGLUTININS EXTRACTION FROM CHLORELLA VULGARIS USING THREE DIFFERENT BUFFERS

CAVALCANTI, V. L. R. ; NASCIMENTO, C. O. ; COSTA, R. M. P. B. ; CARVALHO, J. C. M. ; PORTO, A. L. F. ; BEZERRA, R. P. ; MARQUES, D. A. VIANA ;

Artigo:

Agglutinins are carbohydrates applied as tools in cell biology, immunology, in the study and treatment of cancers, and in a range of areas of biochemical and biomedical research. This study aims to determine the best method of possible hemagglutinating substances extraction from Chlorella vulgaris biomass using three different buffers. The cultured was concentrated by a centrifugation at 4,000 xg for 10 min, lyophilized and extracted under homogenization in pure and in concentrations of 1:5 to 1:20 using the buffers 0.025M Tris-HCl-saline, pH 7.0; 0.02M saline-phosphate, pH 7.4; and 0.1M sodium acetate, pH, 5.5. In microtiter boards, biomass extracts were added to rabbit erythrocytes for hemagglutination activity. Positive results were obtained in Tris-HCl-saline buffer (1:10 and 1:20 dilutions) and sodium acetate buffer (pure extract and dilutions) with hemagglutination titers of 2

Artigo:

Download (PDF)

Palavras-chave:

DOI: 10.5151/chemeng-cobeq2014-0555-24971-151835

Referências bibliográficas
  • [1] BERMEJO ROMAN R, TALAVERA EM, ALVAREZ-PEZ JM. Chromatographic purification and characterization of b-phycoerythrin from Porphyridium cruentum. Semipreparative HPLC separation and characterization of its subunits. Journal of Chromatography A, v. 917, p.135– 45, 200
  • [2] BHAGAVATHY, S.; SUMATHI, P.; BEL, J.; S. Green algae Chlorococcumhumicola-a new source of bioactive compounds with antimicrobial activity. Asian Pacific Journal of Tropical Biomedicine, p. S1-S7, 2011.
  • [3] BISCHOFF, H. W.; BOLD, H. C. Phycological Studies. IV. Some Algae from Enchanted Rock and Related Algae Species. University of Texas Publications number 6318. pp. 1-95, 196
  • [4] BOYD,W.C.; ALMODOVAR, L.R. Andamp; BOYD, L.G. Agglutinins in marine algae for human erythrocytes. Transfusion, 6:82-83, 1966.
  • [5] CHU, C.Y.; LAIO, W.R.; HUANG, R.; LIN, L.P. Hemagglutinating and antibiotic activities of freshwater microalgae. World Journal of Microbiology and Biotechnology, v. 20, p. 817–825, 2004.
  • [6] CHU, C.Y.; HUANG, R.; LIN, L.P. Purification and characterization of a novel haemagglutinin from Chlorella pyrenoidosa. Journal of Industrial Microbiology and Biotechnology, v. 33, p. 967–973, 200
  • [7] CORREIA M.T.S., COELHO L.C.B.B. Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart (Camaratu Bean), Appl Biochem Biotech. 55 (1995) 261-273.
  • [8] COSTA, J.A.V; RADMANN, E.M; CERQUEIRA, V.S; Perfil de ácidos graxos das microalgas Chlorella vulgaris E Chlorella minutissima cultivadas em diferentes condições. Alimentação e Nutrição, Araraquara ,v.17, n.4, p.429-436, out./dez. 2006.
  • [9] DINH, H. L.; HORI, K.; QUANG, N. H. Screening and preliminary characterization of hemagglutinins in Vietnamese marine algae. Journal Applied of Phycology, v. 21, p. 89–97, 200
  • [10] GUEDES, A.C.; AMARO, H.M.; MALCATA, F.X. Microalgae as Sources of Carotenoids. Mar. Área temática: Processos Biotecnológicos 56 Drugs 2011, 9, 625-644.
  • [11] HORI, K., IKEGAMI, S., MIYAZAWA, K. Andamp; ITO, K. Mitogenic and antineoplastic isoagglutinins from the red alga Solieria robusta. Phytochemistry, v. 27(7), p. 2063-2067, 1988.
  • [12] LOURENÇO, S.O. Cultivo de microalgas marinhas – princípios e aplicações. São Carlos: RiMa, 2006.
  • [13] MOSTAFA,S.M. Microalgal Biotechnology: prospects and applications. Plant Science. Chaper 12, 2012. Informations are available at the site: http://dx.doi.org/10.5772/53649.
  • [14] PULZ, O.; GROSS, W. Valuable products from biotechnology of microalgae. Applied Microbiology Biotechnology, v.65, p.635-648, 2004.
  • [15] SAKAMOTO, T.; SHEN, G.; HIGASHI, S.; MURATA, N.; BRYANT, D. A. Alteration of low-temperature susceptibility of the cyanobacterium Synechococcus sp. PCC 7002 by genetic manipulation of membrane lipid unsaturation. Archives of Microbiology, v.169, n. 1, p. 20-28, 1997. http://dx.doi.org/10.1007/s002030050536.
  • [16] SAMPAIO, ALEXANDRE HOLANDA et al. Hemaglutininas de algas marinhas. Revista Brasileira de Fisiologia Vegetal, v. 5, p. 171-177, 1993.
  • [17] SATO, YUICHIRO et al. Purification and characterization of a novel lectin from a freshwater cyanobacterium, Oscillatoria agardhii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, v. 125, n. 2, p. 169-177, 2000.
  • [18] UMA R., SIVASUBRAMANIAN V., S.NIRANJALI DEVARAJ. Evaluation of in vitro antioxidant activities and antiproliferative activity of green microalgae, Desmococcus olivaceous and Chlorococcum humicola. J. Algal Biomass Utln., 2 (3): 82– 93, 2011.
  • [19] UTEX. Disponível em: http://www.sbs.utexas.edu/utex/. Acessado em 13.fev.2013.
  • [20] YAMAGUCHI M, OGAWA T, MURAMOTO K, KAMIO Y, JIMBO M KAMIYA H. Isolation and characterization of mannanbinding lectin from the freshwater cyanobacterium (bluegreen algae) Microcystis viridis. Biochem Biophys Res Comm, 265:703–708, 1999.
Como citar:

CAVALCANTI, V. L. R.; NASCIMENTO, C. O.; COSTA, R. M. P. B.; CARVALHO, J. C. M.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA; "AGGLUTININS EXTRACTION FROM CHLORELLA VULGARIS USING THREE DIFFERENT BUFFERS", p. 784-789 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0555-24971-151835

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações