Abril 2017 vol. 4 num. 1 - V SEQUFES – V Semana da Engenharia Química da Universidade Federal do Espírito Santo

Artigo Completo - Open Access.

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Análise de canabinóides neutros em derivados de Cannabis via GC/MS

Grijó, D. R. ; Cardozo, L. F. ;

Artigo Completo:

Atualmente a medicina tem grande interesse na espécie Cannabis sativa L., especialmente em dois de seus canabinóides neutros (CN): canabidiol (CBD) e 9-tetrahidrocanabinol (9-THC). Em parceria com a Polícia Científica de Curitiba – Brasil, foram feitas análises, via cromatografia gasosa e espectrometria de massas (GC/MS), da composição e o comportamento térmico de CN em derivados dessa espécie, com solvente polar e apolar. Testou-se também, em sementes, uma metodologia industrial de limpeza para remover resíduos de CN aderidos por contato com outras partes da planta. A partir de curvas de calibração, utilizando padrões de CBD, 9-THC e canabinol (CBN), foi possível fazer análises quantitativas da influência térmica e da metodologia de lavagem. Esses e outros canabinóides foram identificados pela razão massa carga (m/z). Foi comprovado que o aquecimento de amostras aumenta o potencial medicinal, mas também transforma o 9-THC em CBN e/ou 8-tetraidrocanabinol (8-THC), e o CBD em canabielsoin (CBE) e/ou 9-THC. O extrato apolar apresentou maior concentração de CN. Demonstrou-se também a diminuição de CN nas sementes pelo método de lavagem industrial, bem como o rendimento em óleo esperado em referências. Com esse trabalho foi possível demonstrar a importância de conhecimento do comportamento dos CN em derivados de Cannabis.

Artigo Completo:

Currently the medicine has great interest in the species Cannabis sativa L., especially in two of its neutral cannabinoids (CN): cannabidiol (CBD) and 9-tetrahydrocanabinol (9-THC). In partnership with the Scientific Policy of Curitiba - Brazil, analyses had been made, with gas chromatography and mass spectrometry (GC/MS), of the composition and the thermal behavior of CN in derivatives of this species, with polar and nonpolar solvent. It was also tested in seeds an industrial cleaning method to remove CN residues adhered by contact with other parts of the plant. From the calibration curves using the standards CBD, 9-THC and canabinol (CBN), it was possible to make quantitative analysis of the thermal influence and the cleaning method. Those and others canabinnoids were identified by mass/charge ratio (m/z). It was proven that the heating samples increases the medicinal potential, but also transforms 9-THC in CBN and/or 8-tetraidrocanabinol (8-THC), and CBD in canabielsoin (CBE) and/or 9-THC. The nonpolar extract has a higher concentration of CN. Cleaning method reduce CN in seeds, as well oil yield expected results. With this work it was possible to demonstrate the importance of knowledge of the CN behavior in Cannabis derivatives.

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Palavras-chave: cannabinoids, gas chromatography, mass spectrometry.,

Palavras-chave: ,

DOI: 10.5151/SEQUFES2016-006

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Como citar:

Grijó, D. R.; Cardozo, L. F.; "Análise de canabinóides neutros em derivados de Cannabis via GC/MS", p. 23-27 . In: . São Paulo: Blucher, 2017.
ISSN 2359-1757, DOI 10.5151/SEQUFES2016-006

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