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This paper explores the impact of furniture and machines on spatial organization and circulation systems in industrial buildings using space syntax analysis. While space syntax research covers various settings, industrial buildings have received limited attention. This study aims to address this gap by examining how machines and furniture influence spatial organization and circulation in two industrial buildings: A Nitrile Glove manufacturing facility and a textile manufacturing factory. The furnished and unfurnished floor plans were analyzed using space syntax software, DepthMapX, with connectivity and agent-based analyses. The results indicate that unfurnished plans have centrally located connectivity values, whereas furnished plans create subspaces with varying connectivity. Agent-based analysis reveals that unfurnished spaces have high density in the center, while furnished spaces distribute density more evenly, resulting in more uniform circulation. This study concludes that industrial building spatial configurations result from a combination of architectural design and the placement of machines and furniture.