Blucher Chemical Engineering Proceedings

Setembro 2018, vol.1, num.5 - XXII Congresso Brasileiro de Engenharia Química
Pôster - Open Access Idioma principal | Segundo principal

CELLULASE PRODUCTION BY Bacillus subtilis

CELLULASE PRODUCTION BY Bacillus subtilis

SILVA, H. N. L; SALOMÃO, G. S; LIRA, T. S; PINOTTI, L. M

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Bacteria who has high growth rate are a promising alternative to cost reduction in cellulase production. This study investigates de cellulose production from Bacillus subtilis using sugarcane bagasse and carboxymetilcellulose as carbon source in submerged fermentation. The factorial planning methodology and the response surface analysis were used to evaluate the effect of the concentration of bagasse (0.5; 1.6 e 2.7%) and carboxymetilcellulose (1.0; 1.5 e 2.0%). The experimental design used has two factors (32 ), resulting in 11 experiments, since three of them were central point replications. It was verified that both substrates concentrations were statistic significant on enzyme production. The enzyme activities varied between 73 UI/L of extract and 145 UI/L at 33ºC for 24h, being the best result obtain in the assay with the higher concentrations of bagasse and CMC. A bioreactor was used which the purpose of analyze the oxygen effect on the enzyme production. In this step, there was an increase on the enzyme production, in 8h of fermentation reached a max activity of 197 UI/L.

Bacteria who has high growth rate are a promising alternative to cost reduction in cellulase production. This study investigates de cellulose production from Bacillus subtilis using sugarcane bagasse and carboxymetilcellulose as carbon source in submerged fermentation. The factorial planning methodology and the response surface analysis were used to evaluate the effect of the concentration of bagasse (0.5; 1.6 e 2.7%) and carboxymetilcellulose (1.0; 1.5 e 2.0%). The experimental design used has two factors (32 ), resulting in 11 experiments, since three of them were central point replications. It was verified that both substrates concentrations were statistic significant on enzyme production. The enzyme activities varied between 73 UI/L of extract and 145 UI/L at 33ºC for 24h, being the best result obtain in the assay with the higher concentrations of bagasse and CMC. A bioreactor was used which the purpose of analyze the oxygen effect on the enzyme production. In this step, there was an increase on the enzyme production, in 8h of fermentation reached a max activity of 197 UI/L.

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DOI: 10.5151/cobeq2018-PT.0907

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Como citar:

SILVA, H. N. L; SALOMÃO, G. S; LIRA, T. S; PINOTTI, L. M; "CELLULASE PRODUCTION BY Bacillus subtilis", p-3434-3437. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-PT.0907

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