Novembro 2015 vol. 1 num. 2 - V SIMPÓSIO DE BIOQUÍMICA E BIOTECNOLOGIA

Artigo Completo - Open Access.

Idioma principal

Continuous Cultivations of the Oleaginous Yeast Lipomyces starkeyi

Anschau, Andréia ; Franco, Telma Teixeira ;

Artigo Completo:

Oleaginous yeasts are microbial factories capable of converting carbohydrates into lipids. The transesterification of these lipids results in the production of biodiesel. The oleaginous yeast Lipomyces starkeyi is an excellent lipid producer. To attain parameters for the understanding of the lipid production process, we performed continuous cultivations using glucose and xylose as carbon sources. The highest overall cell yield of 0.443 g/g and lipid yield of 0.236 g/g was observed at the dilution rate of 0.03 h-1 while the highest cell and lipid productivities were obtained at 0.06 h-1. Gas chromatography analysis of the esterified lipids indicated that the major constituents of this complex are palmitic acid, stearic acid, oleic acid, and linoleic acid with an estimated cetane number (approximately 61) similar to that of palm biodiesel, which is important for biofuel production. These data should be very helpful to develop and design more efficient bioprocesses for microbial lipid production.

Artigo Completo:

Download (PDF)

Palavras-chave: oleaginous yeast, dilution rate, biodiesel,

Palavras-chave: ,

DOI: 10.5151/biochem-vsimbbtec-21648

Referências bibliográficas
  • [1] ANSCHAU, A.; XAVIER, M. C. A.; HERNALSTEENS, S.; FRANCO, T. T. Effect of feeding strategies on lipid production by Lipomyces starkeyi. Bioresour. Technol., v. 157, p. 214–222, 2014.
  • [2] CORADINI, A. L. V.; ANSCHAU, A.; VIDOTTI, A. D. S.; REIS, E. M.; XAVIER, M. C. A.; COELHO, R. S.; FRANCO, T. T. Microorganism for bioconversion of sugar hydrolysates into lipids. In: KAMM, B. (Ed.). Microorganisms in Biorefineries. Berlin Heidelberg: Springer-Verlag, v.26, 2015. cap. 3, p.51-78.
  • [3] FEI, Q.; WEWETZER, S. J.; KUROSAWA, K.; RHA, C.; SINSKEY, A. J. High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose. Process Biochem. , v. In Press, 2015.
  • [4] GILL, C. O.; HALL, M. J.; RATLEDGE, C. Lipid accumulation in an oleaginous yeast (Candida 107) growing on glucose in single stage continuous culture. Appl. Environ. Microbiol., v. 33, n. 2, p. 231-239, 1977.
  • [5] HU, C.; WU, S.; WANG, Q.; JIN, G.; SHEN, H.; ZHAO, Z. K. Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum. Biotechnol. Biofuels, v. 4, p. 25-32, Aug 24 2011.
  • [6] HUANG, C.; ZONG, M. H.; WU, H.; LIU, Q. P. Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour. Technol., v. 100, n. 19, p. 4535-4538, 2009.
  • [7] KOSA, M.; RAGAUSKAS, A. J. Lipids from heterotrophic microbes: Advances in metabolism research. Trends Biotechnol., v. 29, n. 2, p. 53-61, Feb 2011.
  • [8] LIU, Y. F.; MENG, Z.; ZHANG, F. Q.; SHAN, L.; WANG, X. G. Influence of lipid composition, crystallization behavior and microstructure on hardness of palm oil-based margarines. Eur. Food Res. Technol., v. 230, n. 5, p. 759-767, 2010.
  • [9] NORHAIZAN, M. E.; HOSSEINI, S.; GANGADARAN, S.; LEE, S. T.; KAPOURCHALI, F. R.; MOGHADASIAN, M. H. Palm oil: Features and applications. Lipid Technol., v. 25, n. 2, p. 39-42, 2013.
  • [10] NOVICK, A.; SZILARD, L. Description of the chemostat. Science, v. 112, n. 2920, p. 715-716, 1950.
  • [11] PAPANIKOLAOU, S.; AGGELIS, G. Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single stage continuous culture. Bioresour. Technol., v. 82, n. 1, p. 43-49, 2002.
  • [12] RATLEDGE, C.; WYNN, J. P. The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv. Appl. Microbiol., v. 51, p. 1-51, 2002.
  • [13] SHEN, H.; GONG, Z.; YANG, X.; JIN, G.; BAI, F.; ZHAO, Z. K. Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides. J. Biotechnol., v. 168, n. 1, p. 85-89, 20
  • [14] TAPIA, E. V.; ANSCHAU, A.; CORADINI, A. L.; FRANCO, T. T.; DECKMANN, A. C. Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening. AMB Express, v. 2, n. 1, p. 64-71, Dec 5 2012.
  • [15] XAVIER, M. C. A.; FRANCO, T. T. Batch and continuous culture of hemicellulosic hydrolysate from sugarcane bagasse for lipids production. Chem. Eng. Trans., v. 38, 2014.
  • [16] ZHAO, X.; KONG, X. L.; HUA, Y. Y.; FENG, B.; ZHAO, Z. B. Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi. Eur. J. Lipid Sci. Technol., v. 110, n. 5, p. 405-412, 2008.
Como citar:

Anschau, Andréia; Franco, Telma Teixeira; "Continuous Cultivations of the Oleaginous Yeast Lipomyces starkeyi", p. 9-12 . In: In Anais do V Simpósio de Bioquímica e Biotecnologia - VSIMBBTEC [=Blucher Biochemistry Proceedings].. São Paulo: Blucher, 2015.
ISSN 2359-5043, DOI 10.5151/biochem-vsimbbtec-21648

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações