Setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

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CULTIVATION OF Bacillus thuringiensis var. israelensis H14 IN BIOREACTOR FOR BIOLOGICAL CONTROL OF Aedes aegypti LARVAE

MELO, L. F. A. ; CABRAL, A. M. ; MELO, A. C. A. ; MELO-SANTOS, M. A. V. ; FINKLER, L. ; LUNA-FINKLER, C .L. ;

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The aim of this study was the cultivation of Bacillus thuringiensisvar. israelensis (Bti) H14 for the biological control of Aedes aegypti. Bti cultureswere cultivated in a bioreactor, and the growth kinetics, toxin concentration andlarvicidal activity were evaluated. The results showed that the bacterialfermentates produced on average 0.62 g/L of toxin (LC50 2.25 mg/L and LC903.82 mg/L). Upon concentration of the biomass, the toxin yield was almostthree-fold higher (1.64 g/L), also resulting in an increase in the bioactivity(LC50 0.46 mg/L and LC90 0.84 mg/L). The fermentation conditions employedwere suitable for the cultivation of Bti, generating batches with highconcentration of cells, short time required for sporulation and adequateproduction of toxins with confirmed biolarvicidal activity.

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DOI: 10.5151/cobeq2018-PT.0577

Referências bibliográficas
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Como citar:

MELO, L. F. A.; CABRAL, A. M.; MELO, A. C. A.; MELO-SANTOS, M. A. V.; FINKLER, L.; LUNA-FINKLER, C .L.; "CULTIVATION OF Bacillus thuringiensis var. israelensis H14 IN BIOREACTOR FOR BIOLOGICAL CONTROL OF Aedes aegypti LARVAE", p. 2182-2185 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-PT.0577

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