Dezembro 2024 vol. 11 num. 2 - X Simpósio Internacional de Inovação e Tecnologia

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ENZYMATIC IMMOBILIZATION OF Aspergillus LIPASE USING COCOA SHELL AS A SUPPORT MATERIAL AND CHARACTERIZATION OF THE IMMOBILIZED BIOCATALYST

ENZYMATIC IMMOBILIZATION OF Aspergillus LIPASE USING COCOA SHELL AS A SUPPORT MATERIAL AND CHARACTERIZATION OF THE IMMOBILIZED BIOCATALYST

Pita, Bruna Louise de Moura ; Nascimento, Luciana Lordêlo ; Almeida, Lorena Santos de ; Ferreira, Larissa da Silveira ; Teixeira, Fernanda Ribeiro Pitta ; Moreira, Camila de Almeida ; Fricks, Alini Tinoco ;

Completo:

"The use of enzymatic immobilization in agro-industrial waste as support is an interesting biotechnological alternative for managing these dejects in the country. This work aimed to immobilize Aspergillus lipase in cocoa bean shell (CBS) by physical adsorption and covalent bonding, and the biochemical characterization of the immobilized biocatalysts as a function of pH, temperature and operational stability. Covalent bonding was the most efficient technique for recovering enzymatic activity, and the immobilized biocatalysts presented an optimal temperature in the range of 37-50°C, good activity at pH 7-8 and stability for up to six reuse cycles, thus demonstrating excellent potential for industrial applications and relocation of agro-industrial waste"

Completo:

"The use of enzymatic immobilization in agro-industrial waste as support is an interesting biotechnological alternative for managing these dejects in the country. This work aimed to immobilize Aspergillus lipase in cocoa bean shell (CBS) by physical adsorption and covalent bonding, and the biochemical characterization of the immobilized biocatalysts as a function of pH, temperature and operational stability. Covalent bonding was the most efficient technique for recovering enzymatic activity, and the immobilized biocatalysts presented an optimal temperature in the range of 37-50°C, good activity at pH 7-8 and stability for up to six reuse cycles, thus demonstrating excellent potential for industrial applications and relocation of agro-industrial waste"

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Palavras-chave: Immobilization, Enzyme, Waste valorization, Cocoa, Biochemistry,

Palavras-chave: Immobilization, Enzyme, Waste valorization, Cocoa, Biochemistry,

DOI: 10.5151/siintec2024-393429

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Como citar:

Pita, Bruna Louise de Moura; Nascimento, Luciana Lordêlo; Almeida, Lorena Santos de; Ferreira, Larissa da Silveira; Teixeira, Fernanda Ribeiro Pitta; Moreira, Camila de Almeida; Fricks, Alini Tinoco; "ENZYMATIC IMMOBILIZATION OF Aspergillus LIPASE USING COCOA SHELL AS A SUPPORT MATERIAL AND CHARACTERIZATION OF THE IMMOBILIZED BIOCATALYST", p. 1024-1033 . In: . São Paulo: Blucher, 2024.
ISSN 2357-7592, DOI 10.5151/siintec2024-393429

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