Setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

Oral - Open Access.

Idioma principal

EXPRESSION AND PURIFICATION OF A NEW ESTERASE (LIPG7) FROM METAGENOMIC CLONE SIMILAR TO BACTERIAL ESTERASE OF THE Porticoccus hydrocarbonoclasticus

ARAUJO, F. J ; GOMES, B. S. R ; BESSA, C. C ; SOARES-JUNIOR, J. A. O ; HISSA, D. C ; MELO, V. M. M ; , ;

Oral:

Esterases are lipolytic enzymes widely used in industrial applications. This work aimed the overexpression and purification of the esterase LipG7 identified from a metagenomic library constructed from mangrove sediments, which gene sequence shares 80% amino acid identity to 1,4-butanediol diacrylate esterase from the bacterium Porticoccus hydrocarbonoclasticus. LipG7 was expressed in three commercial Escherichia coli strains, Rosetta-gami, ArcticExpress and BL21 and the activity evaluated against 4-nitrophenyl butyrate substract. Recombinante esterase was obtained in soluble form only in Rosetta-gami after treatment with guanidine hydrochloride. It was active against 4-nitrophenyl butyrate at 30 °C with specific activity of 216.3 ± 16.4 U/mg that was significantly enhanced in presence of Mg2+ ion.

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Palavras-chave: Porticoccus hydrocarbonoclasticus,

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DOI: 10.5151/cobeq2018-CO.115

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Como citar:

ARAUJO, F. J; GOMES, B. S. R; BESSA, C. C; SOARES-JUNIOR, J. A. O; HISSA, D. C; MELO, V. M. M; , ; "EXPRESSION AND PURIFICATION OF A NEW ESTERASE (LIPG7) FROM METAGENOMIC CLONE SIMILAR TO BACTERIAL ESTERASE OF THE Porticoccus hydrocarbonoclasticus", p. 4528-4532 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-CO.115

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