Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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FABRICATION, CHARACTERIZATION, AND FUNCTIONALIZATION OF GOLD ELECTRODES WITH POLY(ETHYLENE GLYCOL) FOR BIOSENSING APPLICATIONS

CRULHAS, B. P. ; CASTRO, C. M. ; PEDROSA, V. A. ;

Artigo:

Enzyme-based electrodes are one of the most studied and are applied widely for metabolites detection, like glucose and lactate, byproducts in a variety of diseases, as diabetes, obesity and cancer. The electrodes miniaturization was aided by adaption of semiconductor fabrication processes. But immobilizing distinct biorecognition elements on an electrode array still a challenging process. For this, in our work we developed a multianalyte gold electrode conjugated with Poly(ethylene glycol) (PEG), a widespread polymer used in biomaterials and biotechnology, which provides an excellent matrix for enzymes, and has unique properties, as biocompatible, nontoxic and hydrophilic, creating a robust sensor for biosensing applications. The characterization and functionalization were made by electrochemical assays, using cyclic voltammetry and impedance espectroscopy and the results showed an improvement of the electrode measurements when used the PEG conjugated with ferrocene and enzymes.

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DOI: 10.5151/chemeng-cobeq2014-1012-21665-160517

Referências bibliográficas
  • [1] M. Bui, S. Lee, K. Han, X. Pham, C. Li, J. Choo, G. Seong, Chem. Commun. 2009, 5549.
  • [2] W. Cai, Q. Xu, X. Zhao, J. Zhu, H. Chen, Chem. Mater. 2006, 18, 279.
  • [3] Charnley, M.; Textor, M.; Khademhosseini, A.; Lutolf, M. P. Integr. Biol. 2009, 1, 625−634.
  • [4] W. Chen, C. Li, P. Chen, C. Sun, Electrochim. Acta 2007, 52,2845.
  • [5] Cohen, A.; Kunz, R. Sens. Actuators, B 2000, 62, 23–29.
  • [6] P. D. Drumheller, J. A. Hubbell, J. Biomed. Mater. Res. 1995, 29, 207.
  • [7] Y. Feng, T. Yang, W. Zhang, C. Jiang, K. Jiao, Anal. Chim. Acta 2008, 616, 144.
  • [8] Gatenby, R. A.; Gillies, R. J. Nat. Rev. Cancer 2004, 4, 891–899.
  • [9] Guenat, O. T.; Generelli, S.; de Rooij, N. F.; Koudelka-Hep, M.; Berthiaume, F.; Yarmush, M. L. Anal. Chem. 2006, 78, 7453–7460.
  • [10] Hu, Y.; Zhang, Y.; Wilson, G. S. Anal. Chim. Acta 1993, 1993, 503–511.
  • [11] Ju, H.; Leech, D. J. Chem. Soc., Faraday Trans. 1997, 93, 1371-1375 W. G. Koh, M. Pishko, Sens. Actuators B 2005, 106, 335.
  • [12] Koudelka-Hep, M., De Rooij, N. F., Strike, D. J. In Immobilization of Enzymes and Cells; Guisan, J. M., Ed.; Methods in Biotechnology; Humana Press: Totowa, NJ, 1997; Vol. 1, pp 83-85.
  • [13] W. Lee, R. Scholz, K. Niesch, U. Gosele, Angew. Chem.-Int. Ed. 2005, 44, 6050.
  • [14] Área temática: Processos Biotecnológicos 8Lee, J. Y.; Shah, S. S.; Yan, J.; Howland, M. C.; Parikh, A. N.; Pan, T. R.; Revzin, A. Langmuir 2009, 25, 3880–3886.
  • [15] X. Luo, J. Xu, Q. Zhang, G. Yang, H. Chen, Biosens. Bioelectron. 2005, 21, 190 Malitesta, C.; Palmisano, F.; Torsi, L.; Zambonin, P. G. Anal. Chem. 1990, 62, 2735–2740.
  • [16] Moser, I.; Jobst, G.; Urban, G. Biosens. Bioelectron. 2002, 17, 297– 302.
  • [17] Mugweru, A.; Clark, B. L.; Pishko, M. V. Electroanalysis 2007, 19, 453–458.
  • [18] V. Pardo-Yissar, R. Gabai, A. Shipway, T. Bourenko, I. Willner, Adv. Mater. 2001, 13, 1320.
  • [19] C. P. Quinn, C. P. Pathak, A. Heller, J. A. Hubbell, Biomaterials 1995, 16, 389.
  • [20] Ohara, T. J.; Rajagopalan, R.; Heller, A. Anal. Chem. 1994, 66,2451–2457.
  • [21] O’Rahilly, S.; Vidal-Puig, A. Science 2001, 431, 125–126.
  • [22] Revzin, R. J. Russell, V. K. Yadavalli, W. G. Koh, C. Deister, D. D. Hile, M. B. Mellott, M. V. Pishko, Langmuir 2001, 17, 5440 Revzin, A.; Sirkar, K.; Simonian, A.; Pishko, M. V. Sens. Actuators,B 2002, 81, 359–368.
  • [23] A. Revzin, R. G. Tompkins, M. Toner, Langmuir 2003, 19, 9855.
  • [24] Sirkar, K.; Revzin, A.; Pishko, M. V. Anal. Chem. 2000, 72, 2930– 2936.
  • [25] Spiegelman, B. M.; Filer, J. S. Cell 2001, 104, 531–543.
  • [26] Strike, D. J.; De Rooij, N. F.; Koudelka-Hep, M. In Immobilization of Enzymes and Cells; Guisan, J. M., Ed.; Methods in Biotechnology; Humana Press: Totowa, NJ, 1997; Vol. 1, pp 93-100.
  • [27] Sundberg SA (2000) Curr Opin Biotechnol 11(1):47–53 Y. Xiao, F. Patolsky, E. Katz, J. Hainfeld, I. Willner, Science 2003, 299, 1877.
  • [28] Zhu, H.; Stybayeva, G. S.; Silangcruz, J.; Yan, J.; Ramanculov, E.; Dandekar, S.; George, M. D.; Revzin, A. Anal. Chem. 2009, 81, 8150–8156.
  • [29] Yan, J., Pedrosa, V., Simonian, A.L., Revzin, A. “Immobilizing Enzymes onto Electrode Arrays by Hydrogel Photolithography to Fabricate Multi-Analyte Electrochemical Biosensors” ACS Applied Materials Andamp; Interfaces, 2010, 2, 748-755.
  • [30] Yan, J.,Valber, A., Pedrosa, V., Enomoto, J., Simonian, A., and Revzin, A. “Electrochemical biosensors for on-chip detection of oxidative stress.” Biomicrofluidics, 2011, 5(3), 032008-11.
Como citar:

CRULHAS, B. P.; CASTRO, C. M.; PEDROSA, V. A.; "FABRICATION, CHARACTERIZATION, AND FUNCTIONALIZATION OF GOLD ELECTRODES WITH POLY(ETHYLENE GLYCOL) FOR BIOSENSING APPLICATIONS", p. 1433-1441 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-1012-21665-160517

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