Dezembro 2024 vol. 11 num. 2 - X Simpósio Internacional de Inovação e Tecnologia

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IMMOBILIZATION OF BURKHOLDERIA CEPACIA LIPASE IN AGRO-INDUSTRIAL RESIDUE FROM THE COCOA INDUSTRY AND BIOCHEMICAL CHARACTERIZATION

IMMOBILIZATION OF BURKHOLDERIA CEPACIA LIPASE IN AGRO-INDUSTRIAL RESIDUE FROM THE COCOA INDUSTRY AND BIOCHEMICAL CHARACTERIZATION

Pita, Bruna ; Nascimento, Luciana ; Almeida, Lorena ; Ferreira, Larissa ; Teixeira, Fernanda ; Moreira, Camila ; Fricks, Alini Tinoco ;

Completo:

"Enzymatic immobilization makes industrial processes more productive and cost-effective, and its combination with the reuse of agro-industrial residues offers an alternative for reducing undesired environmental impacts. This work aimed to immobilize Burkholderia cepacia lipase on cocoa bean shell (CBS) using physical adsorption and covalent bonding, and to characterize the biochemical properties of the immobilized biocatalysts in terms of pH, temperature, and operational stability. Covalent bonding was the most efficient technique for recovering enzymatic activity. The immobilized biocatalysts showed optimal pH ranges between 7 and 8.5, low thermal stability, and operational stability between 7 and 9 reuse cycles, demonstrating significant potential for industrial applications."

Completo:

"Enzymatic immobilization makes industrial processes more productive and cost-effective, and its combination with the reuse of agro-industrial residues offers an alternative for reducing undesired environmental impacts. This work aimed to immobilize Burkholderia cepacia lipase on cocoa bean shell (CBS) using physical adsorption and covalent bonding, and to characterize the biochemical properties of the immobilized biocatalysts in terms of pH, temperature, and operational stability. Covalent bonding was the most efficient technique for recovering enzymatic activity. The immobilized biocatalysts showed optimal pH ranges between 7 and 8.5, low thermal stability, and operational stability between 7 and 9 reuse cycles, demonstrating significant potential for industrial applications."

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Palavras-chave: Immobilization; Enzyme; Waste valorization; Cocoa; Biochemistry,

Palavras-chave: Immobilization; Enzyme; Waste valorization; Cocoa; Biochemistry,

DOI: 10.5151/siintec2024-393423

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Como citar:

Pita, Bruna; Nascimento, Luciana; Almeida, Lorena; Ferreira, Larissa; Teixeira, Fernanda; Moreira, Camila; Fricks, Alini Tinoco; "IMMOBILIZATION OF BURKHOLDERIA CEPACIA LIPASE IN AGRO-INDUSTRIAL RESIDUE FROM THE COCOA INDUSTRY AND BIOCHEMICAL CHARACTERIZATION", p. 1015-1023 . In: . São Paulo: Blucher, 2024.
ISSN 2357-7592, DOI 10.5151/siintec2024-393423

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