Setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

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INFLUENCE OF THE APROTIC IONIC LIQUID OF Burkholderia cepacia LIPASE IMMOBILIZED ON HYBRID SUPPORT

MARTINS, S. R. S ; BARBOSA, A. S ; LIMA, A. S ; CABRERA-PADILLA, R. Y ; SOUZA, R. L ; SOARES, C. M. F ;

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Ionic liquids can act as support modifiers and additives in the enzymatic immobilization process, providing more efficient and stable immobilized biocatalysts. In this context, the present work aims to evaluate the influence of aprotic ionic liquids (AIL), ethyl (C2), butyl (C4) e hexyl (C6) of 3-methylimidazolium bis (trifluoromethysulphonyl) imid) in the preparation of hybrid support formed by hydroxybutyrate-hydroxyvalerate (PHBV) and silica for posterior immobilization of Burkholderia cepacia lipase (BCL). Biochemical characterization was evaluted pH (4 - 8), temperature (37 - 75 °C), thermal stability (55 and 65 °C) and operational stability. Immobilized biocatalysts (IB) higher values immobilization yield obtained IB-C4 (171 ± 1%) and IB-C6 (169 ± 3%) higher than the IB-Control (90%). It can be observed that the free and immobilized BCL by covalent binding presented a better hydrolytic activity at neutral pH (7.0) and the temperature of 50 °C, indicating that the modification of the hybrid support with AIL did not interfere in the pH and optimum temperature of the enzyme. In the study of thermal stability, the AIL modified biocatalysts of higher alkyl chains (C4 and C6) were more stable than the control in the higher ranges of the temperatures studied (55 and 65 °C). The reuse of BCL showed similar results for the biocatalysts IB-C4 and IB-C6, which were reused 11 times, maintaining 50% of the initial activity, whereas the control presented only six reuses. Therefore, the use of modified supports with aprotic ionic liquids for enzyme immobilization offers new perspectives for the industry, thus increasing its efficiency, stability and reuse.

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Palavras-chave: hydroxybutyrate-hydroxyvalerate,

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DOI: 10.5151/cobeq2018-PT.0862

Referências bibliográficas
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Como citar:

MARTINS, S. R. S; BARBOSA, A. S; LIMA, A. S; CABRERA-PADILLA, R. Y; SOUZA, R. L; SOARES, C. M. F; "INFLUENCE OF THE APROTIC IONIC LIQUID OF Burkholderia cepacia LIPASE IMMOBILIZED ON HYBRID SUPPORT", p. 3262-3265 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-PT.0862

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