ISOTHERM STUDIES OF ADSORPTION OF Burkholderia cepacia LIPASE ON BIOCHAR FROM GUAVA SEEDS (Psidium guajava L)

ISOTHERM STUDIES OF ADSORPTION OF Burkholderia cepacia LIPASE ON BIOCHAR FROM GUAVA SEEDS (Psidium guajava L)

ALMEIDA, L. C.; LISBOA, M. C.; MENDES, A. A.; FREITAS, L. S.; LIMA, A. S.; SOARES, C. F. M.

Pôster:

Biochar, a product of the pyrolysis of agroindustrial wastes, isrecognized as a multifunctional material. Its use as a support in enzyme immobilizationhas been successfully reported in order to reduce immobilization processes costs and topromote stabilization of enzymes. The objective of this work was to elucidate theadsorption process of lipase from Burkholderia cepacia (BCL) on biochar from guavaseeds by fitting the experimental data to four typical isotherm models such as Langmuir,Sips, Freundlich and Redlich-Peterson. According to results, Sips isotherm model had abetter fit to the experimental data, considering its highest correlation coefficient (R2=0.9879) and the lowest ARE value (0.0103).

Biochar, a product of the pyrolysis of agroindustrial wastes, isrecognized as a multifunctional material. Its use as a support in enzyme immobilizationhas been successfully reported in order to reduce immobilization processes costs and topromote stabilization of enzymes. The objective of this work was to elucidate theadsorption process of lipase from Burkholderia cepacia (BCL) on biochar from guavaseeds by fitting the experimental data to four typical isotherm models such as Langmuir,Sips, Freundlich and Redlich-Peterson. According to results, Sips isotherm model had abetter fit to the experimental data, considering its highest correlation coefficient (R2=0.9879) and the lowest ARE value (0.0103).

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DOI: 10.5151/cobeq2018-PT.0590

Referências bibliográficas

• [1] ALMEIDA LC, BARBOSA AS, FRICKS AT, FREITAS LS, LIMA AS, SOARES CMF, Use of conventional or non-conventional treatments of biochar for lipase Immobilization. Process Biochem., v. 61, p. 124-129, 2017. ALVES MD, ARACRI FM, CREN EC, MENDES AA, Isotherm, kinetic, mechanism and thermodynamic studies of adsorption of a microbial lipase on a mesoporous and hydrophobic resin. Chem. Eng. J., v. 311, p. 1-12, 2017. ES I, VIEIRA JDG, AMARAL AC, Principles, techniques, and applications of biocatalyst immobilization for industrial application. Appl. Microb. Biotechnol., 99, p. 2065-2082, 2015. PODDER MS, MAJUMDER CB, Studies on the removal of As(III) and As(V) through their adsorption onto granular activated carbon/MnFe2O4 composite: isotherm studies and error analysis. J. Composite Interfaces, v. 23, p. 327-372, 2016. TRODLER P, SCHMID RD, PLEISS J, Modeling of solvent-dependent conformational transitions in Burkholderia cepacia lipase. BMC Struct. Biol., v. 9, p. 1-13, 2009. SANTOS RM, SANTOS AO, SUSSUCHI EM, NASCIMENTO JS, LIMA AS, FREITAS LS, Pyrolysis of mangaba seed: Production and characterization of bio-oil. Bioresour. Technol., v. 196, p. 43-48, 2015.

Como citar:

ALMEIDA, L. C.; LISBOA, M. C.; MENDES, A. A.; FREITAS, L. S.; LIMA, A. S.; SOARES, C. F. M.; "ISOTHERM STUDIES OF ADSORPTION OF Burkholderia cepacia LIPASE ON BIOCHAR FROM GUAVA SEEDS (Psidium guajava L)", p-2235-2238. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-PT.0590