Nisin Activity Against Contaminant Bacteria Isolated From Bioethanol Fermentation Tanks

Nisin Activity Against Contaminant Bacteria Isolated From Bioethanol Fermentation Tanks

Maia, Natalia; Rigotti, Rachel; Silva, Renata; Lange, Bruna; Meca, Giuseppe; Macedo, Renata; Luciano, Fernando

Abstract:

Nisin is a bacteriocin (lantibiotic) produced by Lactococcus lactis, which is widely used in the food industry as a natural antimicrobial to avoid the growth of pathogenic and spoilage bacteria.The objective of the present work was to evaluate the antimicrobial activity of nisin to wards Leuconostoc mesenteroides and Lactobacillus fermentum, which are common contaminants in Brazilian bioethanol production plants. These bacteria are normally present in the wort and significantly reduce the sugar-to-ethanol conversion capacity of Saccharomyces cerevisiae. Firstly, the Minimum Inhibitory Concentration (MIC) of nisin was determined for each one of the bacteria in tubes containing CSN broth (supplemented sugarcane broth). Then, a wide range of nisin doses was tested (0.125 – 7,500 ppm) during CSN (10 oBRIX and pH 5,0) fermentation. The broth was co-inoculated with Saccharomyces cerevisiae, Lactobacillus fermentum and Leuconostoc mesenteroides and incubated for 24 h at 30oC. Then, samples from each batch were submitted to microbial count, oBRIX analysis, yeast viability and determination of ethanol content. All tests were performed 3 times in duplicate (n=6). The MIC for both bacteria was 0.75 ppm. The average bacterial population in the control group (non-treated samples) was 10.4 log UFC/mL after the fermentation period. The lowest dose used, 0.125 ppm, did not present significant reduction of the bacterial contamination. However, all other doses presented a drastic of the bacterial population: 4.3 log UFC/mL with 0.375 ppm; 5.8 log UFC/mL with 0.75 ppm (=CIM), 5.7 log UFC/mL with 7.5 ppm, 7.5 log UFC/mL with 750 ppm; and Andamp;gt; 9.4 log CFU/mL with 7,500 ppm. None of the doses tested presented a significant reduction of Saccharomyces cerevisiae population, and the yeast viability was always Andamp;gt; 95%. Groups treated with Andamp;#8805; 0,75 ppm presented lower o BRIX values and higher ethanol concentration than the control group. These results show that nisin may be used as a natural antibacterial agent during the production of bioethanol in order to reduce the population of Lactobacillus fermentum and Leuconostoc mesenteroides, and, consequently, increase the sugar-to-ethanol conversion rate by Saccharomyces cerevisiae.

Nisin is a bacteriocin (lantibiotic) produced by Lactococcus lactis, which is widely used in the food industry as a natural antimicrobial to avoid the growth of pathogenic and spoilage bacteria.The objective of the present work was to evaluate the antimicrobial activity of nisin to wards Leuconostoc mesenteroides and Lactobacillus fermentum, which are common contaminants in Brazilian bioethanol production plants. These bacteria are normally present in the wort and significantly reduce the sugar-to-ethanol conversion capacity of Saccharomyces cerevisiae. Firstly, the Minimum Inhibitory Concentration (MIC) of nisin was determined for each one of the bacteria in tubes containing CSN broth (supplemented sugarcane broth). Then, a wide range of nisin doses was tested (0.125 – 7,500 ppm) during CSN (10 oBRIX and pH 5,0) fermentation. The broth was co-inoculated with Saccharomyces cerevisiae, Lactobacillus fermentum and Leuconostoc mesenteroides and incubated for 24 h at 30oC. Then, samples from each batch were submitted to microbial count, oBRIX analysis, yeast viability and determination of ethanol content. All tests were performed 3 times in duplicate (n=6). The MIC for both bacteria was 0.75 ppm. The average bacterial population in the control group (non-treated samples) was 10.4 log UFC/mL after the fermentation period. The lowest dose used, 0.125 ppm, did not present significant reduction of the bacterial contamination. However, all other doses presented a drastic of the bacterial population: 4.3 log UFC/mL with 0.375 ppm; 5.8 log UFC/mL with 0.75 ppm (=CIM), 5.7 log UFC/mL with 7.5 ppm, 7.5 log UFC/mL with 750 ppm; and Andamp;gt; 9.4 log CFU/mL with 7,500 ppm. None of the doses tested presented a significant reduction of Saccharomyces cerevisiae population, and the yeast viability was always Andamp;gt; 95%. Groups treated with Andamp;#8805; 0,75 ppm presented lower o BRIX values and higher ethanol concentration than the control group. These results show that nisin may be used as a natural antibacterial agent during the production of bioethanol in order to reduce the population of Lactobacillus fermentum and Leuconostoc mesenteroides, and, consequently, increase the sugar-to-ethanol conversion rate by Saccharomyces cerevisiae.

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DOI: 10.5151/foodsci-microal-339

Como citar:

Maia, Natalia; Rigotti, Rachel; Silva, Renata; Lange, Bruna; Meca, Giuseppe; Macedo, Renata; Luciano, Fernando; "Nisin Activity Against Contaminant Bacteria Isolated From Bioethanol Fermentation Tanks", p-137-138. In: Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene [=Blucher Food Science Proceedings, v.1, n.1]. São Paulo: Blucher, 2014.
ISSN 2359201X, DOI 10.5151/foodsci-microal-339