PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS

PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS

Riquelme, Bibiana; Estrada, Ezequiel; Castellini, Horacio; Acosta, Andrea; Chinelatto, Alejandro; Tac, Ivan; Borraz, Javier; Tullio, Liliana Di; Galassi, Mariel E.

Artigo completo:

INTRODUCTION Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008).

INTRODUCTION Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008).

Palavras-chave:

DOI: 10.5151/biofisica2019-30

Referências bibliográficas

• [1] ADAMS, F. et al. Biochemical Storage Lesions Occurring in Nonirradiated and Irradiated Red lood Cells: A Brief Review. BioMed Research International, Article ID 968302, 2015.
• [2] ALBEA, B. et al. New instrument for easy determination of rheological parameters of erythrocytes. Biochimica Clinica 37, S437, 2013.
• [3] ANAND, A.J. et al. Radiation-induced red cell damage: role of reactive oxygen species. Transfusion Vol. 37, 160-165, 1997.
• [4] BASKURT, O. et al. Handbook of Hemorheology and Hemodynamics. 1st ed. Amsterdam: IOS Press, 2007.
• [5] KHALID A.Z. et al. Nanoestructural Changes in the Cell Membrane of Gamma-Irradiated Red Blood Cells. Indian J Hematol Blood Tranfus, Vol. 33, 109-115, 2017.
• [6] KIM, Y.-K. et al. Susceptibility of oxidative stress on red blood cells exposed to gamma rays: Hemorheological evaluation. Clinical Hemorheology and Microcirculation Vol. 40, 315-324, 2008.
• [7] PELSZYNSKI, M.; MOROFF, G.; LUBAN, N.; TAYLOR B.; QUINONES R. Effect of gamma irradiation of red blood cell units on T-cell inactivation as assessed by limiting dilution analysis: implications for preventing transfusion-associated graft-versus-host disease. Blood 83:1683-1689, 1994.
• [8] RIQUELME, B.; VALVERDE, J.; RASIA, R.J. Complex viscoelasticity of normal and lectin treated erythrocyte using laser diffractometry. Biorheology 35, 325–334, 1998.
• [9] RIQUELME, B.; VALVERDE, J.; RASIA, R.J. Optical method to determine the complex viscoelasticity parameters of human red blood cells. In Megha Singh et al. (eds), Medical Diagnostic Techniques and Procedures. New Delhi: Narosa Publishing House, pp. 249–255, 1999.
• [10] RIQUELME B. et al. Study of polycation effects on erythrocyte agglutination mediated by anti-glycophorins using microscopic image digital analysis. In Grzymala R and Haeberle O (eds), Biophotonics and New Therapy Frontiers. Bellingham, WA: SPIE, 2006.
• [11] TODERI, M.A.; CASTELLINI, H.; RIQUELME, B. Simplified variant of an optical chip to evaluate aggregation of red blood cells, Proc SPIE 9531, Biophotonics South America, 95313X, 2015.
• [12] YOUSUF, R. et al. Effects of gamma irradiation on red blood cells at different storage period: A comparative study. Bangladesh Journal of Medical Science, Vol. 17, 2018.

Como citar:

Riquelme, Bibiana; Estrada, Ezequiel; Castellini, Horacio; Acosta, Andrea; Chinelatto, Alejandro; Tac, Ivan; Borraz, Javier; Tullio, Liliana Di; Galassi, Mariel E.; "PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS", p-107-108. In: Anais do Encontro Anual da Biofísica 2019. São Paulo: Blucher, 2019.
ISSN 2526-6071, DOI 10.5151/biofisica2019-30