Blucher Chemical Engineering Proceedings

Fevereiro 2015, vol.1, num.2 - XX Congresso Brasileiro de Engenharia Química
Artigo - Open Access Idioma principal | Segundo principal

PRODUÇÃO DE FENOL-OXIDASES PELO FUNGO Pleurotus sajor-caju PS-2001 EM BIORREATOR AIRLIFT DE CIRCULAÇÃO INTERNA

PRODUÇÃO DE FENOL-OXIDASES PELO FUNGO Pleurotus sajor-caju PS-2001 EM BIORREATOR AIRLIFT DE CIRCULAÇÃO INTERNA

BETTIN, F.; COUSSEAU, F.; MARTINS, K.; ZACCARIA, S.; CUNHA, G. G. S.; SILVEIRA, M. M. da; DILLON, A. J. P.

Artigo:

Fenol-oxidases são enzimas com elevado potencial de aplicação industrial por serem não específicas com relação ao substrato e capazes de oxidar compostos com estruturas semelhantes à lignina. Neste estudo, avaliou-se a produção de fenol-oxidases de Pleurotus sajor-caju PS-2001 em biorreator airlift de circulação interna utilizando diferentes volumes de meio de cultivo (4, 4,5 e 5 L) e vazões de ar (1, 1,5 e 2 L.min

Fenol-oxidases são enzimas com elevado potencial de aplicação industrial por serem não específicas com relação ao substrato e capazes de oxidar compostos com estruturas semelhantes à lignina. Neste estudo, avaliou-se a produção de fenol-oxidases de Pleurotus sajor-caju PS-2001 em biorreator airlift de circulação interna utilizando diferentes volumes de meio de cultivo (4, 4,5 e 5 L) e vazões de ar (1, 1,5 e 2 L.min

Download (PDF)

Palavras-chave:

DOI: 10.5151/chemeng-cobeq2014-2041-16196-135356

Referências bibliográficas
  • [1] BARR, D.P.; AUST, S.D. Mechanism white rot fungi use to degrade pollutants. Environ. Sci. Technol., v. 28, p. 78-87, 1994.
  • [2] BETTIN, F.; MONTANARI, Q.; CALLONI, R.; GAIO, T.A.; SILVEIRA, M.M.; DILLON, A.J.P. Production of laccases in submerged process by Pleurotus sajor-caju PS-2001 in relation to carbon and organic nitrogen sources, antifoams and Tween 80. J. Ind. Microbiol. Biotechnol., v. 36, p. 1-9, 2009.
  • [3] Área temática: Processos Biotecnológicos 6BETTIN, F.; ROSA, L.O.; MONTANARI, Q.; CALLONI, R.; GAIO, T.A.; MALVESSI, E.; SILVEIRA, M.M.; DILLON, A.J.P. Growth, kinetics, production, and characterization of extracellular laccases from Pleurotus sajor-caju PS-2001. Process Biochem., v. 46, p. 758-764, 2011.
  • [4] BOURBONNAIS, R.; PAICE, M.G. Veratryl alchool oxidases from the lignin-degrading basidiomycete Pleurotus sajor-caju. Biochem. J., v. 255, p. 445-450, 1988.
  • [5] CANET, R.; BIRNSTINGL, J.G.; MALCOLM, D.G.; LOPEZ-REAL, J.M.; BECK, A.J. Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by native microflora and combinations of white-rot fungi in a coal-tar combinated soil. Bioresour. Technol., v. 76, p. 113-117, 2001.
  • [6] CHISTI, Y. Airlift bioreactors. London: Elsevier Science Publishers, 1989.
  • [7] CONFORTIN, F.G.; MARCHETTO; R.; BETTIN, F.; CAMASSOLA, M.; SALVADOR, M.; DILLON, A.J.P. Production of Pleurotus sajor-caju strain PS-2001 biomass in submerged culture. J. Ind. Microbiol. Biotechnol., v. 35, p. 1149-1155, 2008.
  • [8] COUTO, S.R.; HERRERA, J.L.T. Industrial and biotechnological applications of laccases: a review. Biotechnol. Adv., v. 24, p. 500-513, 2006.
  • [9] DHAWAN, S.; LAL, R.; HANSPAL, M.; KUHAD, R.C. Effect of antibiotics on growth and laccase production from Cyathus bulleri and Pycnoporus cinnabarinus. Bioresour. Technol., v. 96, p. 1415-1418, 2005.
  • [10] DOMÍNGUEZ, A.; GÓMEZ, J.; LORENZO, M.; SANROMÁN, A. Enhanced production of laccase activity by Trametes versicolor immobilized into alginate beads by the addition of different inducers. World J. Microbiol. Biotechnol., v. 23, p. 367-373, 2007.
  • [11] FONTANA, R.C.; POLIDORO, T.A.; SILVEIRA, M.M. Comparison of stirred tank and airlift bioreactors in the production of polygalacturonases by Aspergillus oryzae. Bioresour. Technol., v. 100, p. 4493-4498, 2009.
  • [12] FONTANA, R.C.; SILVEIRA, M.M. Production of polygalacturonases by Aspergillus oryzae in stirred tank and internal- and external-loop airlift reactors. Bioresour. Technol., v. 123, p. 157-163, 2012.
  • [13] FREITAS, C.; TEIXEIRA, J.A. Oxygen mass transfer in a high solids loading three-phase internal-loop airlift reactor. Chem. Eng. J. v. 84, p. 57-61, 2001.
  • [14] GILL, P.K.; ARORA, D.S. Effect of culture conditions on manganese peroxidase production and activity by some white rot fungi. J. Ind. Microbiol. Biotechnol., v. 30, p. 28-33, 2003.
  • [15] HEINZKILL, M.; BECH, L.; HALKIER, T.; SCHNEIDER, P.; ANKE, T. Characterization of laccases and peroxidases from wood-rotting fungi (Family Coprinaceae). Appl. Environ. Microbiol., v. 64, p. 1601-1606, 1998.
  • [16] KUWAHARA, M.; GLENN, J.K.; MORGAN, M.A.; GOLD, M.H. Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett., v. 169, p. 247-250, 1984.
  • [17] LIN, J.; HAN, M.; WANG, T.; ZHANG, T.; WANG, J.; JIN, Y. Influence of the gas distributor on the local hydrodynamic behavior of an external loop airlift reactor. Chem. Eng. J., v. 102, p. 51-59, 2004.
  • [18] LIU, J.; CAI, Y.; LIAO, X.; HUANG, Q.; HAO, Z.; HU, M.; ZHANG, D.; LI, Z. Efficiency of laccase production in a 65-L air-lift reactor for potential green industrial and environmental application. J. Cleaner Production, v. 39, p. 154-160, 2013.
  • [19] Área temática: Processos Biotecnológicos 7MANDELS, M.; REESE, E.T. Induction of cellulase in Trichoderma viride as influenced by carbon source and metals. J. Bacteriol., v. 73, p. 269-278, 1957.
  • [20] MÁRQUEZ-ROCHA, F.J.; GUILLÉN, G.K.; SÁNCHEZ, J.E.; VÁZQUEZ-DUHALT, R. Growth characteristics of Pleurotus ostreatus in bioreactors. Biotechnol. Tech., v. 13, p. 29-32, 1999.
  • [21] MERCHUK, J.C.; ASENJO, J.A. Fundamentals of bioreactor design. Bioprocess Technol., v. 21, p. 139-205, 1995.
  • [22] MIKIASHVILI, N.; WASSER, S.P.; NEVO, E.; ELISASHVILI, V. Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity. World J. Microbiol. Biotechnol., v. 22, p. 999-1002, 2006.
  • [23] MILLER, G.L. Use of dinitrosalicilic acid reagent for determination of reducing sugar. Anal. Chem., v. 31, p. 426-428, 1959.
  • [24] MIN, K.L.; KIM, Y.H.; KIM, Y.W.; JUNG, H.S.; HAH, Y.C. Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis. Arch. Biochem. Biophys., v. 392, p. 279-286, 2001.
  • [25] MUNARI, F.M.; GAIO, T.A.; DILLON, A.J.P. Phenol degradation and colour removal in submerged culture of Pleurotus sajor-caju with paper mill effluents. Biocatal. Biotransform., v. 25, p. 24-28, 2007.
  • [26] RANCAÑO, G.; LORENZO, M.; MOLARES, N.; COUTO, S.R.; SANROMÁN, M.A. (2003). Production of laccase by Trametes versicolor in an airlift fermentor. Process Biochem., v. 39, p. 467-473, 2003.
  • [27] RITTER, C.E.T.; FONTANA, R.C.; CAMASSOLA, M.; SILVEIRA, M.M.; DILLON, A.J.P. The influence of sorbitol on the production of cellulases and xylanases in an airlift bioreactor. Bioresour. Technol., v. 148, p. 86-90, 2013.
  • [28] RODRÍGUEZ COUTO, S.; RODRÍGUEZ, A.; PATERSON, R.R.M.; LIMA, N.; TEIXEIRA, J.A. Laccase activity from the fungus Trametes hirsuta using an air-lift bioreactor. Lett. Appl. Microbiol., v. 42, p. 612-616, 2006.
  • [29] SAPARRAT, M.C.N.; MARTÍNEZ, M.J.; CABELLO, M.N.; ARAMBARRI, A.M. Screening for ligninolytic enzymes in autochthonous fungal strains from Argentina isolated from different substrata. Rev. Iberoam. Micol., v. 19, p. 181-185, 2002.
  • [30] SCHMITT, S.; SOUZA, R.; BETTIN, F.; DILLON, A.J.P.; VALLE, J.A.B.; ANDREAUS, J. Decolorization of aqueous solutions of disperse textile dyes by oxidoreductases. Biocatal. Biotransform., v. 30, p. 48-56, 2012.
  • [31] TIEN, M.; KIRK, T.K. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc. Natl. Acad. Sci. USA, v. 81, p. 2280-2284, 1984.
  • [32] VIAL, C.; PONCIN, S.; WILD, G.; MIDOUX, N. Experimental and theoretical analysis of the hydrodynamics in the riser of an extemal loop airlift reactor. Chem. Eng. Sci., v. 57, p. 4745-4762, 2002.
  • [33] WOLFENDEN, B.S.; WILLSON, R.L. Radical-cations as reference chromogens in the kinetic studies of one-electron transfer reactions: pulse radiolysis studies of 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate). J. Chem. Soc. Perkin Trans. II, v. 02, p. 805-812, 1982.
  • [34] Apoio: FAPERGS, CAPES, CNPq e UCS.
Como citar:

BETTIN, F.; COUSSEAU, F.; MARTINS, K.; ZACCARIA, S.; CUNHA, G. G. S.; SILVEIRA, M. M. da; DILLON, A. J. P.; "PRODUÇÃO DE FENOL-OXIDASES PELO FUNGO Pleurotus sajor-caju PS-2001 EM BIORREATOR AIRLIFT DE CIRCULAÇÃO INTERNA", p-2815-2822. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-2041-16196-135356

últimos 30 dias

54
downloads

180
visualizações

794
indexações