QUANTIFICATING THE OPHTHALMIC IRRITATION TEST OF PROTICIONIC LIQUIDS USING IMAGEJ AND PHOTOSKAPE

QUANTIFICAÇÃO DO TESTE DE IRRITAÇÃO OFTÁLMICA DE LÍQUIDOS IÔNICOS PRÓTICOS USANDO IMAGEJ E PHOTOSKAPE

Andrade, Rebecca da Silva; Veloso, Gabriela Brasil Romão; ChiariAndreo, Bruna Galdorfini; Duro, Miguel Angel Iglesias

Artigo completo:

The HET-CAM (Hen‟s Egg Chorioallantoic Membrane) assay is aqualitative alternative method to the in vivo Draize Rabbit Eye test to assess theirritancy potential of chemicals. In this work, for the first time the ophthalmic irritationof 13 different protic ionic liquids has been evaluated using ImageJ and PhotoSkapeimage processing programs to analyze the results of the HET-CAM assay. Theirritation potential of a substance can be quantified using these computational toolsby observing blood vessel changes like lysis, hemorrhage and coagulation. Inconclusion, the modification of the established HET-CAM assay made it possibleto determine the damage to minute blood vessels, highlighting the low-irritantprofile of some studied protic ionic liquids to the ocular tissues.

O ensaio HET-CAM (Membrana Corioalantóica do Ovo de Galinha) é um método alternativo qualitativo para o teste in vivo de Draize para avaliar o potencial de irritação de produtos químicos. Neste trabalho, pela primeira vez, a irritação oftálmica de 13 líquidos iônicos próticos (LIPs) diferentes foi avaliada usando os programas de processamento de imagem ImageJ e PhotoSkape para analisar os resultados do ensaio HET-CAM. O potencial de irritação de uma substância pode ser quantificado usando essas ferramentas computacionais, observando as alterações dos vasos sanguíneos, como lise, hemorragia e coagulação. Em conclusão, a modificação deste ensaio tornou possível determinar o dano aos vasos sanguíneos diminutos, destacando o perfil de baixa irritação dos LIPs para os tecidos oculares.

Palavras-chave:

DOI: 10.5151/siintec2020-QUANTIFICATINGTHE

Referências bibliográficas

• [1] ÁLVAREZ, V.H., Iglesias, M., Dosil, N., Gonzalez-Cabaleiro, R., Martin-Pastor, M., Mattedi, S., Navaza, J. M. Brønsted ionic liquids for sustainable processes: synthesis and physical properties, J. Chem. Eng. Data, 55 (2010) 625–632.
• [2] 2 ADAWIYAH, N., Moniruzzaman, M., Hawatulaila, S., Goto, M. Ionic liquids as a potential tool for drug delivery systems, Med. Chem. Commun. 7 (2016) 1881–1897.
• [3] 3 HIJO, A.A.C.T., Maximo, G.J., Costa, M.C., Batista, E.A.C., Meirelles, A.J.A. Applications of ionic liquids in the food and bioproducts industries, ACS Sustain. Chem. Eng. 4 (2016) 5347–5369, https://doi.org/10.1021/acssuschemeng.6b00560.
• [4] 4 ANDRADE, R.S., Torres, D., Ribeiro, F.R., Chiari-Andréo, B.G., Oshiro, J.A. Jr., Iglesias, M. Sustainable cotton dyeing in nonaqueous medium applying protic ionic liquids. ACS Sustain. Chem. Eng. 5 (2017) 8756–8765.
• [5] 5 RANKE, J., Stolte, S., Störmann, R., Arning, J., Jastorff, B. Design of sustainable chemical products – the example of ionic liquids, Chem. Rev. 107 (2007) 2183–2206, https://doi.org/10.1021/cr050942s.
• [6] 6 KUMAR, R.A., Papaïconomou, N., Lee, J.M., Salminen, J., Clark, D.S., Prausnitz, J.M. In vitro cytotoxicities of ionic liquids: effect of cation rings, functional groups, and anions, Environ. Toxicol. 24 (2009) 388–395. https://doi.org/10.1002/tox.20443.
• [7] 7 OLIVEIRA, M.V.S., Vidal, B.T., Melo, C.M., Miranda, R.C.M., Soares, C.M.F., Coutinho, J.A.P., Ventura, S.P.M., Mattedi, S., Lima, A. S. (Eco)toxicity and biodegradability of protic ionic liquids, Chemophere. 147 (2016) 460–466,
• [8] 8 KAUSHIK, N.K., Attri, P., Kaushik, N., Choi, E. H. Synthesis and antiproliferative activity of ammonium and imidazolium ionic liquids against T98G brain cancer cells, Molecules. 17 (2012) 13727–13739. https://doi.org/10.3390/molecules171213727.
• [9] 9 HWANG, J., Park, H., Choi, D.W., Nam, K.T., Lim, K.M. Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models, Toxicol. in vitro. 46 (2018) 194–202.
• [10] 10 PÉREZ, S.A., Montalbán, M.G., Carissimi, G., Licence, P., Víllora, G. In vitro cytotoxicity assessment of monocationic and dicationic pyridinium-based ionic liquids on HeLa, MCF-7, BGM and EA.hy926 cell lines,J. Hazard. Mater., (2019) 121513. https://doi.org/10.1016/j.jhazmat.2019.121513.
• [11] 11 ARUNKUMAR, R., Abraham, A.N., Shukla, R., Drummond, C.J., Greaves, T.L. Cytotoxicity of protic ionic liquids towards the HaCat cell line derived from human skin,J. Mol. Liquids.314 (2020) 113602. https://doi.org/10.1016/j.molliq.2020.113602.
• [12] 12 ENVIRONMENTAL PROTECTION AGENCY (EPA), Alternative Test Methods and Strategies to Reduce Vertebrate Animal Testing. https://www.epa.gov/assessingand-managing-chemicals-under-tsca/alternative-test-methods-and-strategies-reduce, 2018 (accessed 02 August 2020).
• [13] 13 EUROPEAN CHEMICAL AGENCY (ECHA), Advice on skin and eye irritation testing helps reduce animal tests, ECHA/NA/16/28. https://echa.europa.eu/-/adviceon-skin-and-eye-irritation-testing-helps-reduce-animal-tests, 2016 (accessed 02 August 2020).
• [14] 14 ORGANIZATION FOR ECONOMIC CO-OPERATION AND DEVELOPMENT (OECD) TG 263. OECD guidelines for the testing of chemicals test, no. 263: Guidance Document on an Integrated Approach on Testing and Assessment (IATA) for Serious Eye Damage and Eye irritation.
• [15] 15 MACKENZIE, B., Kay, G., H. Matthews, K. H., Knott, R. M., Cairns, D., International Journal of Pharmaceutics, 490, 1-8, 2015.

Como citar:

Andrade, Rebecca da Silva ; Veloso, Gabriela Brasil Romão; ChiariAndreo, Bruna Galdorfini ; Duro, Miguel Angel Iglesias ; "QUANTIFICATING THE OPHTHALMIC IRRITATION TEST OF PROTICIONIC LIQUIDS USING IMAGEJ AND PHOTOSKAPE", p-208-216. In: Anais do VI Simpósio Internacional de Inovação e Tecnologia. São Paulo: Blucher, 2020.
ISSN 23577592, DOI 10.5151/siintec2020-QUANTIFICATINGTHE