Blucher Chemical Engineering Proceedings

Setembro 2018, vol.1, num.5 - XXII Congresso Brasileiro de Engenharia Química
Pôster - Open Access Idioma principal | Segundo principal

REUSE OF CANDIDA ANTARTICA LIPASE B IMMOBILIZED ONTO IRON MAGNETIC NANOPARTICLES FOR SYNTHESIS OF FLAVOR ESTERS

REUSE OF CANDIDA ANTARTICA LIPASE B IMMOBILIZED ONTO IRON MAGNETIC NANOPARTICLES FOR SYNTHESIS OF FLAVOR ESTERS

MONTEIRO, R. R. C; FEITOSA, M. R. C; MOREIRA, K. S; SANTOS, J. C. S; SOUZA, M. C. M

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Enzymes are biocatalysts that allow natural catalysis under mild reaction conditions and are highly selective. In order to increase enzyme stability and facilitate its recovery and reuse, enzymes are immobilized onto magnetic nanoparticles. In this context, this study aims to analyze the maintenance of the catalytic activity of Candida antartica Lipase B immobilized onto magnetic nanoparticles applied to the production of methyl butyrate and ethyl butyrate through esterification. Analyzing the effects of temperature, the best conversion rate was 78.48% for ethyl butyrate at 35 °C, while the conversion rate for methyl butyrate was 80.67% at a substrate concentration of 0.5 mol/L. Analyzing the molar ratio (acid: alcohol), the best conversion rate (44.71%) was obtained for ethyl butyrate using a molar ratio of 1:1.

Enzymes are biocatalysts that allow natural catalysis under mild reaction conditions and are highly selective. In order to increase enzyme stability and facilitate its recovery and reuse, enzymes are immobilized onto magnetic nanoparticles. In this context, this study aims to analyze the maintenance of the catalytic activity of Candida antartica Lipase B immobilized onto magnetic nanoparticles applied to the production of methyl butyrate and ethyl butyrate through esterification. Analyzing the effects of temperature, the best conversion rate was 78.48% for ethyl butyrate at 35 °C, while the conversion rate for methyl butyrate was 80.67% at a substrate concentration of 0.5 mol/L. Analyzing the molar ratio (acid: alcohol), the best conversion rate (44.71%) was obtained for ethyl butyrate using a molar ratio of 1:1.

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DOI: 10.5151/cobeq2018-PT.0916

Referências bibliográficas
  • [1] ADRIANO W, SILVA J, GIORDANO R, GONÇALVES L. Stabilization of penicillin g acylase by
  • [2] immobilization on glutaraldehyde-activated chitosan. Bra. J. of Chem. Eng., v. 22, p. 529–538,
  • [3] 2005.
  • [4] ANDRADE L, REBELO L, NETTO C, TOMA H. Kinetic resolution of a drug precursor by
  • [5] Burkholderia cepacia lipase immobilized by different methodologies on superparamagnetic
  • [6] nanoparticles. J. of Mol. Cat. B: Enz., v. 66 , p. 55–62, 2010.
  • [7] HASAN F, SHAH A, HAMMED A. Industrial applications of microbial lipase. Enzyme and
  • [8] Microbial Technology. Elsevier, v. 39, p. 235–251, 2006.
  • [9] LEI L, BAI Y, LI Y, YI L, YANG Y, XIA C. Study on immobilization of lipase onto magnetic
  • [10] microspheres with epoxy groups. Elsevier, v. 21, p. 252–258, 2009.
  • [11] KISS M, SEFANOVITS-BÁNYAI É, TOTH A, BOROSS L. Extractive synthesis of ethyl-oleate
  • [12] using alginate gel co-entrapped yeast cells and lipase enzyme. Eng. in Life Sci, v. 4, p. 460–464,
  • [13] 2004.
  • [14] NETTO C, ANDRADE L, TOMA H. Enantioselective transesterification catalysis by Candida
  • [15] antarctica lipase immobilized on superparamagnetic nanoparticles. Tetra: Asy., v. 20, p. 2299–2304,
  • [16] 2009.
  • [17] RODRIGUES D, MENDES A, ADRIANO W, GONÇALVES L, GIORDANO R. Multipoint
  • [18] covalent immobilization of microbial lipase on chitosan and agarose activated by different methods.
  • [19] J. of Mol. Cat. B: Enz., v. 51, p. 100–109, 2008.
  • [20] SOUZA MCM. Imobilização de Lipase de Candida Antarctica do Tipo B em Nanopartículas
  • [21] Magnéticas Visando a Aplicação na Síntese de Ésteres. 2013. 87 f. Tese (Doutorado em Engenharia
  • [22] Química). Universidade Federal do Ceará, Fortaleza/ Ceará, 2013.
  • [23] WISEMAN A. Handbook of Enzyme Biotechnology. Londres: Ellis Horwood, 1995.
Como citar:

MONTEIRO, R. R. C; FEITOSA, M. R. C; MOREIRA, K. S; SANTOS, J. C. S; SOUZA, M. C. M; "REUSE OF CANDIDA ANTARTICA LIPASE B IMMOBILIZED ONTO IRON MAGNETIC NANOPARTICLES FOR SYNTHESIS OF FLAVOR ESTERS", p-3470-3473. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-PT.0916

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