Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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1 EXTRACTION OF AGGLUTININS FROM Arthrospira platensis BIOMASS IN THREE DIFFERENT BUFFERS

LIMA, K. L. ; NASCIMENTO, C. O . ; COSTA, R. M. P. B. ; PORTO, A. L. F. ; BEZERRA, R. P. ; MARQUES, D. A. VIANA ;

Artigo:

Arthrospira platensis contains large amounts of proteins, vitamins, lipids and pigments with various biological functions, such as antitumor, antiviral, antimicrobial activities. The aim of this work was detected the presence of agglutinins in Arthrospira platensis biomass extracted in three different buffers. The harvested cells were concentrated by a centrifugation at 4,000 xg for 10 min, lyophilized and extracted under sonication in pure and in concentrations of 1:5 to 1:20 of 0.025M Tris-HCl-saline, pH 7.0; 0.02M saline-phosphate, pH 7.4; and 0.1M sodium acetate, pH, 5.5 buffers. For hemagglutination activity tests, in microtiter boards, extracts were added to rabbit erythrocytes. Positive results were obtained for hemagglutination activity only in saline-phosphate buffer (1:5, 1:10, and 1:20 dilutions) and sodium acetate buffer (1:5, and 1:10 dilutions) with titers of 2

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DOI: 10.5151/chemeng-cobeq2014-0592-24800-171812

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Como citar:

LIMA, K. L.; NASCIMENTO, C. O .; COSTA, R. M. P. B.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA; "1 EXTRACTION OF AGGLUTININS FROM Arthrospira platensis BIOMASS IN THREE DIFFERENT BUFFERS", p. 888-893 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0592-24800-171812

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