Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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EXTRACTION OF ANTIBACTERIAL SUBSTANCES FROM ArtrhospiraplatensisBIOMASS AGAINST ANTIBIOTIC-RESISTANT Staphylococcus sp. ISOLATED FROM BOVINE MASTITIS

NETO, N. S. O ; MOTA, R. ; CARVALHO, J. C. M ; PORTO, A. L. F. ; MARQUES, D. A. VIANA ; BEZERRA, R. P. ;

Artigo:

Mastitis is an inflammation of the udder which may be caused by bacteria and cause economic losses by produce harmful substances that result in inflammation, reduced milk production, and altered milk quality. Arthrospiraplatensis extract in two different solvents, water (W) and sodium acetate buffer (SAB), was tested for its antibacterial activity against 14 differents antibiotic-resistant Staphylococcus sp. isolated from bovine mastitis. Both Arthrospiraplatensis extract exhibited antimicrobial activity against all Staphylococcus sp., but SAB was most effective than W. The minimum inhibitory concentration of the A. platensis extracts at 100mg/ml in SAB was obtained in 29% of isolated and in W was 21%. More than 50% of inhibition was observed at 6 mg/ml in SAB and 12 mg/ml in W. Results suggest that Arthorspiraplatensis extracts in SAB has the potential to be evaluated as an alternative or adjunct to antibiotics as intramammary infusion to treat bovine mastitis.

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DOI: 10.5151/chemeng-cobeq2014-0591-24803-171692

Referências bibliográficas
  • [1] ABD EL-BAKY, H.H., EL BAZ, F.K., EL-BAROTY, G.S. Over-production of Lipid Rich in g Linolenic Acid by Blue Green Alga Spirulina maxima and its Inhibitory Effect on Carcinoma Cells. Ad. Food Sci., v.4, p. 206-212, 2006.
  • [2] BENNETT, R.H.; CHRISTIANSEN, K.; CLIFTON-HADLEY, R.S.; Estimating the costs associated with endemic diseases of dairy cows. J. Dairy Res. 66, P. 455-459, 1999.
  • [3] DEINHOFER M; PERNTHANER A.Staphylococcus spp. as mastitis-related pathogens in goat milk.Vet. Microbiol., v. 43, p. 161-166, 1995.
  • [4] FERRAZ, C. A. M. Produção de Spirulina maxima: Influência de ecofatores e uso de um sub produto da indústria alcooeira. Rev. Microbiol., v. 17,p. 70-74, 1986.
  • [5] GUEDES, A.C., BARBOSA, C.R., AMARO, H.M., PEREIRA, C.I. Andamp; MALCATA, F.X.. Microalgal and cyanobacterial cell extracts for use as natural antibacterial additives against food pathogens. Int. J. Food. Sci. Technol., v .46, p. 862–870, 2011.
  • [6] Área temática: Processos Biotecnológicos 5KAUSHIK, P.; CHAUHAN, A. In vitro antibacterial activity of laboratory grown culture of Spirulinaplatensis. Indian J. M. Microbiol., v.48, p.348-52, 2008.
  • [7] MCDOUGALL, S.; HUSSEIN, H.; PETROVKI, K. Antimicrobial resistance in Staphylococcus aureus, Streptococcus uberis and Streptococcus dysgalactiae from dairy cows with mastitis.New Zealand Vet J. v. 62, p. 68-76, 2014.
  • [8] MEDINA-JARITZ, N.B; PEREZ-SOLIS, D.R.; RUILOBA DE LEON, F.S.L; OLVERA-RAMIREZ, R.. Antimicrobial activity of aqueous and methanolic extracts from Arthrospira maxima. In: Science Against Microbial Pathogens: Communicating Current Research and Technological Advances (edited by A. Mendez-Vilas). Microbiology Series, v. 1, p. 1267–1271, 2011.
  • [9] NAJDENSK, H.M. I; GIGOVA, G.L; LLIEVE, I.I; PILARSK, P.S; LUKAVISKY, J; TSVETKOVA, I.V. Antibacterial and antifungal activities of selected microalgae and cyanobacteria. Int. J. Food. Sci. Technol, v. 48, p. 1533- 1540, 2013.
  • [10] NCCLS.Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 6th ed. NCCLS document M7-A6. NCCLS, Wayne, Pa. 2003.
  • [11] RADOSTITS, O.M., BLOOD, D.C., GAY, C.C., Mastitis. In: Veterinary Medicine. Bailliëre Tindal, London, UK, pp. 563-627,1994.
  • [12] SARADA, D.V.L.; KUMAR, C.S; RENGASAMY, R. Purified C-phycocyanin from Spirulinaplatensis (Nordstedt) Geitler: a novel and potent agent against drug resistant bacteria. World Journal of Microbio and Biotech v. 27, p. 779–783, 2011.
  • [13] SCHLÖSSER U.G. Sammlung von Algenkulturen. Ber Deutsch Bot Ges, v. 95, p. 181–276, 1982.
  • [14] SEENIVASAN, R.; INDY, H.; ARCHANA, G.; GEETHA, S.The antibacterial activity of some marine algae from south east coast of India.J Pharm Res, v.3, p.1907-1912, 2010.
  • [15] SHARAF, M.; AMARA, A; ABOUL-ENEIN, A; HELMI, S; BALLOT, A; SCHNITZLER, P. Antiherpetic efficacy of aqueous extracts of the cyanobacterium Arthrospira fusiformis from Chad. Pharmazie, v. 68, p .376-380. 2013.
  • [16] UMA, R.; SIVASUBRAMANIAN, V.; NIRANJALI, D. S. Preliminary phycochemical analysis and in vitro antibacterial screening of green micro algae, Desmococcusolivaceous,Chlorococcumhumicola and Chlorella vulgaris. J Algal Biomass Utln v.2, p.74-81, 2011.
  • [17] UTEX. Disponível em: http://www.sbs.utexas.edu/utex/. Acess 13.feb. 2011.
  • [18] VONSHAK, A., Spirulina: growth, physiology and biochemistry. In Spirulinaplatensis (Arthrospira): physiology, cell-biology and biotechnology. Vonshak, A. (Ed.), Taylor Andamp; Francis, London, p. 43-65, 1997.
  • [19] WELLS, S.J.; OTT, S.L.; HILBERG SEITZINGER, A., Key Health Issues for Dairy cattle – new and old. Symposium: Emerging health issues. J. Dairy Sci. 81, P. 3029-3035, 1998.
  • [20] Área temática: Processos Biotecnológicos 6YASUHARA-BELL, J; YANG, Y ; BARLOW R; TRAPIDO-ROSENTHAL, H; LU, In vitro evaluation of marine-microorganism extracts for anti-viral activity. Virol J., v. 7,p. 182 , 2010.
  • [21] ZARROUK, C., Contributionál’étuded’unecyanophycée.Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthése de Spirulina maxima, Ph.D. Thesis, Ecole des Hautes Etudes emSante Publique, Universitéde Paris , 1966
Como citar:

NETO, N. S. O; MOTA, R.; CARVALHO, J. C. M; PORTO, A. L. F.; MARQUES, D. A. VIANA; BEZERRA, R. P.; "EXTRACTION OF ANTIBACTERIAL SUBSTANCES FROM ArtrhospiraplatensisBIOMASS AGAINST ANTIBIOTIC-RESISTANT Staphylococcus sp. ISOLATED FROM BOVINE MASTITIS", p. 881-887 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0591-24803-171692

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