Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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PURIFICATION AND CHARACTERIZATION OF A NOVEL PROTEASE WITH FIBRINOLYTIC ACTIVITY FROM Mucor subtilissimus UCP 1262

SALES, A. E. ; NASCIMENTO, T. P. ; COSTA, R. M. P. B ; PORTO, C. S. ; CAMPOS-TAKAKI, G. M. de ; PORTO, A. L. F. ; PORTO, T. S. ;

Artigo:

A novel protease with fibrinolytic activity was purified from MucorsubtilissimusUCP 1262usinga two-step purification protocol.The enzyme was pre-purified using cetonic precipitation and adsorbed by ion exchange chromatography on DEAE-sephadex G50. System two-dimensional electrophoresis (2DE) coupled to SDS-PAGE showed a single protein band of15.3kDa approximatelyand isoeletric focusing point of 3.9, exhibiting a nature as an acidic enzyme.The activity was suppressed by Co

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DOI: 10.5151/chemeng-cobeq2014-0802-23696-139130

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Como citar:

SALES, A. E.; NASCIMENTO, T. P.; COSTA, R. M. P. B; PORTO, C. S.; CAMPOS-TAKAKI, G. M. de; PORTO, A. L. F.; PORTO, T. S.; "PURIFICATION AND CHARACTERIZATION OF A NOVEL PROTEASE WITH FIBRINOLYTIC ACTIVITY FROM Mucor subtilissimus UCP 1262", p. 1216-1223 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0802-23696-139130

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